Process for the purification of serum albumin

Details Process for the purification of serum albumin Process for the purification of serum albumin

1997-03-06

2000-11-21

Guzo, David

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Albumin

530363, 530413, 530416, 530417, C07K 14765, C07K 118, C07K 122, C07K 136

Patent

active

061505048

ABSTRACT:
Recombinantly produced serum albumin is purified in a series of steps, optionally by incubation with an anion-exchange adsorbent, followed by affinity chromatography employing a hydrophobic solid phase and using a water-soluble lipid anion as desorbens in the aqueous phase. The immobile phase comprises a carrier coupled to a 2-mercapto or 2-hydroxy alkanoic acid.

REFERENCES:
patent: 4086222 (1978-04-01), Lindquist et al.
patent: 4180672 (1979-12-01), Kurozumi et al.
patent: 4228154 (1980-10-01), Fisher et al.
patent: 5250663 (1993-10-01), Tenold
Wichman et al "Purification of Human Serum Albumin By Affinity Chromatography" Biochim Biophys Acta 372 218-224, 1974.
White et al. "Principles of Biochemistry" p. 581, 1978.
Harris et al. "Protein Purification Methods" p. 245-246, 1989.
Scouten "Affinity Chromatography" p. 48-49, 1981.
Kragh-Hansen, "Molecular Aspects of Ligand Binding to Serum Albumin", Pharmacol. Rev. (1981) 33:17-53.
Peters, "Serum Albumin", Adv. Prot. Chem. (1985) 37:161-245.
Wilchek et al., "Affinity Cromatography", Methods in Enzymology (1984) 104:3.
Stoltz et al., "A Pilot Scale Chromatographic Purification of Albumin From Human Plasma", Bio-Sciences (1987) 6:(4):103-106.
Yang and Tsao, "Affinity Chromatography", Ad. Biochem. Engin. (1982) 25:1942.

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