Process for the purification of serum albumin

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Albumin

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530363, 530413, 530416, 530417, C07K 14765, C07K 118, C07K 122, C07K 136

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active

061505048

ABSTRACT:
Recombinantly produced serum albumin is purified in a series of steps, optionally by incubation with an anion-exchange adsorbent, followed by affinity chromatography employing a hydrophobic solid phase and using a water-soluble lipid anion as desorbens in the aqueous phase. The immobile phase comprises a carrier coupled to a 2-mercapto or 2-hydroxy alkanoic acid.

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Harris et al. "Protein Purification Methods" p. 245-246, 1989.
Scouten "Affinity Chromatography" p. 48-49, 1981.
Kragh-Hansen, "Molecular Aspects of Ligand Binding to Serum Albumin", Pharmacol. Rev. (1981) 33:17-53.
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Wilchek et al., "Affinity Cromatography", Methods in Enzymology (1984) 104:3.
Stoltz et al., "A Pilot Scale Chromatographic Purification of Albumin From Human Plasma", Bio-Sciences (1987) 6:(4):103-106.
Yang and Tsao, "Affinity Chromatography", Ad. Biochem. Engin. (1982) 25:1942.

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