Process for the purification of proteins using acidic polysaccha

Details Process for the purification of proteins using acidic polysaccha Process for the purification of proteins using acidic polysaccha

1986-02-06

1987-05-19

Schain, Howard E.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Separation or purification

426656, 426657, 435190, 435192, 530387, 530415, 530421, 530832, 530833, A23J 120

Patent

active

046670181

ABSTRACT:
A process for the purification of proteins from a fluid medium wherein said proteins have an isoelectric pH higher than the pH of said fluid medium, said process comprising passing the liquid medium over acidic polysaccharide gel particles of at least 0.5 mm in the shortest dimension, and recovering the desired proteins from the gel particles by elution with an aqueous salt solution having ions concentration of at least 5 grams per liter.

REFERENCES:
patent: 2161861 (1939-06-01), Gerlough
patent: 2607768 (1952-08-01), McCready et al.
patent: 3069327 (1962-12-01), Eldridge et al.
patent: 3252961 (1966-05-01), Rodgers et al.
patent: 3404142 (1968-10-01), Shank et al.
patent: 3842062 (1974-10-01), Eastman
patent: 4229342 (1980-10-01), Mirabel
patent: 4436658 (1984-03-01), Peyrouset et al.

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