Process for the purification of proteins

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Glycoprotein – e.g. – mucins – proteoglycans – etc.

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530412, 530413, 530414, 530416, 530417, C07K 114, C07K 14505

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active

059817160

ABSTRACT:
The present invention is directed to a simple and efficient process for the recovery of a biologically active glycoprotein from a biological fluid containing it.
In a preferred embodiment, it comprises:

REFERENCES:
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patent: 5362855 (1994-11-01), Garlick et al.
patent: 5478754 (1995-12-01), Brandt et al.
Desnoyers et al.; Interaction of a Novel Class of Phospholipid-Binding Proteins of Bovine Seminal Fluid with Different Affinity Matrices; Arch. Biochem. Biophys., 1993, 305/2 341-349 (Abstract).
Williams et al.; Fractionation of Membrane Proteins on Phenylboronic Acid-Agarose; Biochem. J., 1982, 205, 167-171.
Gaudreault et al., Glycoprotein Nature of Glycosidases from Leaves of Pisum sativumL.; J. Exp. Botany, 34/146, 1145-1154.
De Cristofaro et al.; Human Platelet Glycocalicin Purification by Phenyl Boronate Affinity Chromatography Coupled to Anion-Exchange High-Performance Liquid Chromatography; J. Chromatography, 426, 1988, 376-380.
Ducrocq et al., `Separation of Nonenzymatically Glycosylated Proteins by Phenylboronate Affinity Chromatography`, Protides Biol. Fluids vol. 33, pp. 651-654, 1985.
File Caplus on STN No. 1991:551765. Wasley et al. `The Importance of N- and O-Linked Oligosaccharides Fro the Biosynthesis and in Vitro and in Vivo Biologic Activities of Erythropoietin`, Blood, vol. 77, No. 12, pp. 2624-32. (abstract only) 1991.

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