Process for the purification of insulins by chromatography

Details Process for the purification of insulins by chromatography Process for the purification of insulins by chromatography

1991-09-03

1993-09-14

Russel, Jeffrey E.

Chemistry: natural resins or derivatives; peptides or proteins;

Peptides of 3 to 100 amino acid residues

Insulin; related peptides

530344, C07K 114, C07K 740

Patent

active

052450082

ABSTRACT:
A process for the purification of insulins and insulin derivatives by chromatography in aqueous, buffered solvents which contain water-miscible organic solvents, on lipophilically modified silica gel, is described, wherein the aqueous, buffered solvents contain zwitterions, or the pH of the solvent mixture is in the vicinity of the isoelectric point of the insulin or insulin derivative to be purified and zwitterions are present.

REFERENCES:
patent: 4129560 (1978-12-01), Zoltobrocki
patent: 4601852 (1986-07-01), Obermeier et al.
patent: 4677192 (1987-06-01), Obermeier et al.
Journal of Chromatography, vol. 256, issued 1983, Parman, "Comparison of Carboxymethyl-Cellulose . . . ", pp. 293-301.
Chemical Abstract 88:177,670m, Shataeva et al., "Thermodynamics of Polyfunctional Interchanges during Ionic Exchange", (1975).
Welinder et al., "Reversed-Phase High-Performance Liquid Chromatography of Insulin: Resolution and Recovery in Relation to Column Geography and Buffer Components", J. Chromatography, vol. 361, pp. 357-367 (1986).

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