Process for the purification of dextran-sucrase

Details Process for the purification of dextran-sucrase Process for the purification of dextran-sucrase

1984-05-03

1986-05-27

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

435814, 435816, 435822, C12N 910, C12R 101

Patent

active

045915636

ABSTRACT:
The invention is directed to a process for the purification of the dextran-sucrase produced by Leuconostoc mesenteroides bacteria.
The process according to the invention consists of adding to the culture medium, that contains the extra-cellular enzyme and dextran, a quantity of a PEG type polyether so that, in the medium appear two non miscible phases, that are thus maintained under stirring in order to obtain a good contact. Thereafter the lower dextran phase is separated from the upper polyether phase in order to provide a dextran-sucrase enriched enzymatic preparation.
The purified enzyme can be used in the synthesis processes of the dextrans possessing specific molecular weights for certain applications.

REFERENCES:
patent: 4144130 (1979-03-01), Kula et al.
patent: 4508825 (1985-04-01), Kim et al.
Federation Proceedings Abstracts, vol. 40, No. 6, Abstract 672 (1981).

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