Chemistry: analytical and immunological testing – Peptide – protein or amino acid
Patent
1989-07-10
1991-07-09
Lacey, David L.
Chemistry: analytical and immunological testing
Peptide, protein or amino acid
435 2, 436827, 436821, 436825, 436174, 436175, 436178, 436501, 436 63, G01N 30100
Patent
active
050305786
ABSTRACT:
C1-inhibitor (C1-Inh), the major regulatory protein of the classical pathway of complement activation, can be purified in a new, simplied three step procedure which includes PEG fractionation, jacalin-agarose chromatography and hydrophobic interaction chromatography on phenyl-Sepharose which takes advantage of the marked hydrophilicity of the inhibitor. This procedure has major advantages over those which have been the most frequently used. This method may be highly adaptable to bulk purification for clinical use or for performing analytical or functional studies on genetically or pathologically altered C1-Inh from clinical specimens.
REFERENCES:
patent: 4642284 (1987-02-01), Cooper et al.
patent: 4960712 (1990-10-01), Theofilopoulous et al.
Pillatte, Y. et al., J. of Immunological Methods, 120(1) 1989, 37-44, A Simplified Procedure for C1-Inh. Purification, A Novel Use for Jacalin-Agarose.
Frank Michael M.
Fries Louis F.
Hammer Carl H.
Pilatte Yannick M.
Lacey David L.
Singla Abanti B.
The United States of America as represented by the Secretary of
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