Process for the purification and sterilization of acidophilic bi

Details Process for the purification and sterilization of acidophilic bi Process for the purification and sterilization of acidophilic bi

1975-04-28

1976-10-19

Shapiro, Lionel M.

Textiles: fiber preparation

195 17, 195 18, 195124, 260112R, 536 9, 536 10, 536 15, 536 17, 260559AT, 21 58, C07G 702, C07G 7026

Patent

active

039869270

ABSTRACT:
Methods of sterilizing biologicals in which the biologicals are acidified to a pH of not higher than about 1.0, preferably 0.75 to about 0.5, and the acidified biologicals are stored at cold temperatures for a perod of time sufficient to inactivate microbial flora contained therein but not the biological itself, for example, in the range of about 0.degree. to about 10.degree. C for 1-24 hours or longer. Precipitates formed during the storing are clarified and the biologicals are then neutralized, preferably to a pH of from about 7.0 to about 8.0, or that pH at which the biological is best stored, e.g., trypsin at pH 3-4. The methods include clarifying biologicals of precipitates formed within the first two to three hours and then continuing the storing and again clarifying the biologicals of precipitates.
Microbial flora and numerous contaminated proteins contained in the biologicals are removed resulting in completely sterilized biologicals retaining initial activity or titer. If desired, the clarified biologicals may be stored in acidic state since advantageously they reman biologically stable at ambient temperatures when stored at low pH levels, and then neutralized shortly before dispensing. A number of examples is set forth.

REFERENCES:
patent: 2801956 (1957-08-01), Baumgarten et al.
patent: 3037973 (1962-06-01), Sarcona
patent: 3923600 (1975-12-01), Yoshimura et al.

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