Process for the purification and recovery of formate-dehydrogena

Details Process for the purification and recovery of formate-dehydrogena Process for the purification and recovery of formate-dehydrogena

1986-07-30

1989-11-07

Wax, Robert A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

435183, 435188, 435921, C12N 902, C12R 172

Patent

active

048792349

ABSTRACT:
The invention relates to a process for the purification and, if desired, recovery of formate-dehydrogenase (FDH) from Candida boidinii, in which FDH is subjected to a phase distribution in an aqueous 2-phase system that contains a triazine dyestuff that is bonded to an inert water-soluble polymer.

REFERENCES:
patent: 3133001 (1964-05-01), Muset
patent: 4229529 (1980-10-01), Michal et al.
Watson, David H., Michael J. Harvey and Peter D. G. Dean, "The Selective Retardation of NADP.sup.+ -Dependent Dehydrogenases by Immobilized Procion Red HE-3B", Biochem. J., vol. 173 (1978), pp. 591-596.
Kroner, K. H., H. Husteadt, & M. R. Kula, "Evaluation of Crude Dextran as Phase-Forming Polymer for the Extraction of Enzymes in Aqueous Two Phase Systems in Large Scale", Biotechnology & Bioengineering, vol. 24 (1982), pp. 1015-1045.
Kroner, K. H., H. Hustedt, S. Granda, & M. R. Kula, "Technical Aspects of Separation Using Aqueous Two-Phase Systems in Enzyme Isolation Processes", Biotech & Bioengineering, vol. 20 (1978), pp. 1967-1988.
Kroner, K. H., H. Schutte, W. Stach & M. R. Kula, "Scale-Up of Formate Dehydrogenase by Partition", J. Chem. Tech. Biotechnol., vol. 32 (1982), pp. 130-137.
Hustedt, H., K. H. Kroner, W. Stach, & M. R. Kula, "Procedure for the Simultaneous Large-Scale Isolation of Pullulanase and 1,4-.alpha.-Glucan Phosphorylase from Klebsiella pneumoniae Involving Liquid-Liquid Separation", Biotech & Bioengineering, vol. 20 (1978) pp. 1988-2005.

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