Process for the production of unfused IGF-II protein in E. coli

Details Process for the production of unfused IGF-II protein in E. coli Process for the production of unfused IGF-II protein in E. coli

1990-11-21

1993-05-11

Hill, Jr., Robert J.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 691, 43525233, 4353201, 935 38, 530303, 530324, C12N 1517, C12N 1518, C12N 121

Patent

active

052100286

ABSTRACT:
A process for the preparation of a recombinant IGF-II (rIGF-II) without a covalently attached foreign protein moiety and without N-terminal attached methionine or a derivative of methionine or of a salt of said IGF-II, rIGF-II produced by said method, hybrid vectors comprising DNA encoding said rIGF-II, hosts transformed with said vectors, and a process for the isolation of said rIGF-II from the host cell and refolding it into a biologically active form.

REFERENCES:
patent: 2586170 (1952-02-01), Lawrenz
patent: 2807905 (1957-10-01), Ford
patent: 2807906 (1957-10-01), Mun
patent: 2809460 (1957-10-01), Taylor
patent: 3144728 (1964-08-01), Stevens
patent: 3156064 (1964-11-01), Czirr
patent: 4408411 (1983-10-01), Skarnells
patent: 4536984 (1985-08-01), Kowal
patent: 4758512 (1988-07-01), Goldberg
Hammarberg, B. et al., PNAS, 86:4367-4371, Jun. 1989.
Rhee, H. et al., Journal of Biotechnology, 13:293-304, 1990.
Prokop, A. et al. (ed.), Recombinant DNA Technology and Applications, McGraw-Hill, Inc., New York, 1991, pp. 285, 290-291, 299-305.
Hummel, M. et al., Eur. J. Biochem., 180:555-561, 1989.
Rinderknecht and Humbel, FEBS letters, 89:283-286 (1978).
Smith et al., J. Biol. Chem., 264:9314-9321 (1989).
Buell et al., Nucleic Acids Res., 13:1923-1938 (1985).
Amann et al., Gene, 69:301-315 (1988).

Affiliated with

Also associated with

Rating

Process for the production of unfused IGF-II protein in E. coli does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Process for the production of unfused IGF-II protein in E. coli, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for the production of unfused IGF-II protein in E. coli will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1349693