Process for the production of thin sections of biological tissue

Details Process for the production of thin sections of biological tissue Process for the production of thin sections of biological tissue

1983-10-14

1986-05-13

Naff, David M.

Drug, bio-affecting and body treating compositions

Radionuclide or intended radionuclide containing; adjuvant...

Molecular bilayer structure

435 1, 427 4, G01N 106, A01N 100, A01N 102, A01N 300

Patent

active

045885796

ABSTRACT:
The present invention provides a process for the production of thin sections of biological tissue, especially for transmission electron microscopy. In the process a fixed tissue sample to be investigated is dehydrated by infiltration of a hardenable medium miscible with water and subsequently embedded in casting resin, whereafter thin sections are cut from the cast body resulting after hardening of the casting resin. Infiltration is carried out by warming the fixed tissue sample in a mixture of formaldehyde, melamine and water. During warming, the mixture forms water soluble monomeric methylolmelamines which infiltrate the tissue and undergo polycondensation to form water-insoluble polymeric methylolmelamine condensates in the tissue. The casting resin is preferably a melamine resin, and the resin is preferably prepared using a methylolmelamine ether solution containing polyethylene glycol.

REFERENCES:
patent: 3257279 (1966-06-01), Schain
patent: 3527863 (1970-09-01), Weichselbaum
patent: 3624197 (1971-11-01), Schain
patent: 3961097 (1976-06-01), Gravlee, Jr.
patent: 4334844 (1982-06-01), Tanaka
patent: 4497792 (1985-02-01), Gindler
The Condensed Chemical Dictionary, 8th ed., VMR Co., N.Y., 1971, pp. 48, 548 & 915.

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