Process for the production of solution stable alpha-amylase and

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Stablizing an enzyme by forming a mixture – an adduct or a...

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435202, 435222, 435836, C12N 996, C12N 928, C12N 956, C12R 110

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047242086

ABSTRACT:
This invention contemplates a novel process for the preparation of solution stable alpha-amylase obtained from Bacillus licheniformis, and to the high potency liquid enzyme product prepared by this process. Typically, the enzyme-containing solution from a fermentation is concentrated and starch is added to the concentrate. Alternatively, a precipitation agent such as salt is added to the solution, and a cake containing the enzyme precipitates. The cake is then contacted with an aqueous solution containing starch to extract the enzyme out of the cake to provide a stable liquid enzyme product.

REFERENCES:
patent: 3272717 (1966-09-01), Fukumoto et al.
patent: 3661786 (1972-05-01), Desforges
patent: 4519934 (1985-05-01), Eilertsen et al.
Translation of Biokhimiya, vol. 44, No. 6, pp. 1084-1092, Jun. 1979.

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