Process for the production of recombinant polypeptides

Details Process for the production of recombinant polypeptides Process for the production of recombinant polypeptides

1995-06-06

1998-05-05

Degen, Nancy

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4353201, 536 2351, C12P 2102, C07H 2104, C12N 1570

Patent

active

057472904

ABSTRACT:
A process for producing recombinant polypeptides having porcine growth hormone activity, by culturing unicellular organisms containing synthetic DNA sequences coding for such polypeptides and expressing and recovering the polypeptides is described. The recombinant polypeptides may be used in animals in veterinary compositions as effective substitutes for natually derived porcine growth hormone. Recombinant DNA processes for the preparation of the unicellular organisms are also described.

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Itoh et al., DNA 3(2), pp. 157-165 (1984), Efficient Expression in Escherichia coli of a Mature and Modified Human Interferon-.beta..sub.1.
Windass et al., NAR 10(21), pp. 6639-6657 (1982), "The Construction of a Synthetic Escherichia coli trp promoter and its use in the Expression of Synthetic . . . Gene".
DeBoer et al., DNA 2(3), pp. 231-235 (1983), "Portable Shine-Dalgarno Regions: A System for a Systematic Study of Defined Alterations of Nucleotide Sequences . . . Sites".
Scherer et al. (1980), Nucl. Acids Res. 8(17): 3895-3907.
Roberts et al. (1979), Proc. Natl. Acad. Sci USA 76(2): 760-764.
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Yarranton et al. (1984), Gene 28: 293-300.

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