Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound
Patent
1979-12-05
1982-04-20
Shapiro, Lionel M.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing oxygen-containing organic compound
435136, 435849, 435930, C12P 748, C12P 740
Patent
active
043260306
ABSTRACT:
Process for the production of citric acid, characterized in that E. Coli KG 93, F.sup.- is cultivated in a first step for 15-24 hours at a temperature of 20.degree.-37.degree. C. and a pH of 5.0-7.5 on a substrate consisting of whey permeate to which has been added phosphates in a content of 0.8-1.6 g/l and nitrates in a content of 0.8-1.2 g/l or a corresponding quantity of urea, that H. Wickerhamii CBS 4308 in a second step is cultivated for 20-26 hours at a temperature of 15.degree.-35.degree. C. and a pH of 4.5-6.5 on the cultivating solution from the first step, whereupon citric acid is obtained from the cultivating solution in a way known per se.
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Diamond Shamrock Corporation
Shapiro Lionel M.
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