Process for the production of proteins and the production of...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

Reexamination Certificate

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C435S383000, C435S288400, C435S070100, C435S395000

Reexamination Certificate

active

10399954

ABSTRACT:
Described is a novel process for the production of at least one protein of interest by secretion of the protein of interest from a pro- or eukaryotic host cell in a compartment system, which host cell is stably expressing a secretion system and capable of heterologous secretion of the protein of interest and which compartment system has at least a first and a second compartment and wherein the host cell is located in the first compartment and wherein the first and second compartment are separated from each other by a barrier, wherein the barrier is permeable for the secreted protein of interest, but not permeable for the host cell.

REFERENCES:
patent: 5437998 (1995-08-01), Schwarz et al.
patent: 6544788 (2003-04-01), Singh
patent: WO 01/79849 (2001-10-01), None
Koronakis, Vassilis, et al., “Energetically distinct early and late stages of HlyB/HlyD-dependent secretion across bothEscherichia colimembranes”. The EMBO Journal, 10: 3263-3272, 1991.
Walter, Gerald, et al., “Protein arrays for gene expression and molecular interaction screening”. Current Opinion in Microbiology, 3: 298-302, 2000.
Lueking, Angelika, et al., “Protein Microarrays for Gene Expression and Antibody Screening”. Analytical Biochemistry, 270: 103-111, 1999.
Gentschev, Ivaylo, et al., “Development of antigen-delivery systems, based on theEscherichia colihemolysin secretion pathway”. Gene, 179: 133-140, 1996.
Emili, Alia Qureshi, et al., “Large-scale functional analysis using peptide or protein arrays”. Nature Biotechnology: 18, 393-397, Apr. 2000.

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