Process for the production of inoculum for anaerobic fermentatio

Details Process for the production of inoculum for anaerobic fermentatio Process for the production of inoculum for anaerobic fermentatio

1984-09-07

1988-06-21

Hazel, Blondel

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

435 86, C12N 120, C12P 1942

Patent

active

047525843

ABSTRACT:
A fermentation process is disclosed for the preparation of an inoculum, suitable for batchwise, semicontinuous, or continuous production of coenzyme B.sub.12 under septic conditions. The fermentation process begins with a broth consisting of methanol, cornsteep liquor hydrolysate or heat-treated corn slop, ammonium bicarbonate, magnesium chloride, cobalt chloride, 5,6-dimethyl-benzimidazole and sodium bisulfite used for anaerobic, mesophilic, septic fermentation production of coenzyme B.sub.12 with anaerobic, digested sewage sludge. Fermentation is carried out over several steps to provide an inoculum containing an anaerobic, mesophilic, methane-producing, new mixed micropopulation, containing bacteria of each of the types deposited at the Hungarian National Collection of Medical Bacteria, National Institute of Hygiene, under numbers 00076, 00079, and 00272.

REFERENCES:
patent: 3964971 (1976-06-01), Johan et al.
patent: 3979259 (1976-09-01), Johan et al.
patent: 3996105 (1976-12-01), Harrison et al.
patent: 4119492 (1978-10-01), Kojima et al.
patent: 4430429 (1984-02-01), Zeikus et al.
Harrison, "Mixed Cultures in Industrial Fermentation Processes", Adv. Appl. Microbiol., vol. 24, (1978), pp. 129-164.

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