Process for the production of high purity thrombin

Details Process for the production of high purity thrombin Process for the production of high purity thrombin

1991-01-23

1992-09-29

Naff, David M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

424 9464, C12N 974, A61K 37547

Patent

active

051513552

ABSTRACT:
A liquid thrombin preparation is prepared by reacting a unit of prothrombin with less than 5 units of thromboplastin in the presence of calcium, contacting the resultant thrombin with a phosphate buffer, and diluting and filtering the suspension. The filtrate is then applied sequentially to an anion-exchange agarose column and a cation-exchange agarose column and the thrombin fraction is step-wise eluted from the latter column with phosphate buffered saline. Liquid thrombin preparations thereby obtained have specific activities greater than 1,600 U/mg and can be used in haemostasis.

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patent: 4696812 (1987-09-01), Silbering et al.
patent: 4736018 (1988-04-01), Reutelingspurger
patent: 4965203 (1990-10-01), Silbering et al.

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