Process for the production of glycerides with lipases

Details Process for the production of glycerides with lipases Process for the production of glycerides with lipases

2005-06-14

2005-06-14

Carr, Deborah D. (Department: 1621)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing oxygen-containing organic compound

C554S169000

Reexamination Certificate

active

06905850

ABSTRACT:
The invention provides a process for producing 2-monoglycerides (2-MG) or triglycerides (TG), particularly triglycerides having polyunsaturated fatty acid (PUFA) residues in the sn-2 positions, at an extremely high purity and a high efficiency.The 2-MG is produced by conducting alcoholysis of a starting TG with a first 1,3-lipase. Then, the TG is produced by introducing fatty acid residues to the 1- and 3-positions of the produced 2-MG by using a second 1,3-lipase. The fatty acid residue in the 2-posiiton is preferably a residue of DHA, EPA or ARA. The “first lipase” refers to one which is selective for the 1- and 3-positions and can act even on a long-chain fatty acid in alcoholysis, while the “second lipase” refers to one which is selective for the 1- and 3-positions and can produce a TG through the reaction of a 2-MG with a fatty acid ester or a free fatty acid.

REFERENCES:
R. Irimescu et al., Utilization of Reaction Medium-Dependent Regiospecificity ofCandida antarcticaLipase (Novozym 435) for the Synthesis of 1,3-Dicapryloyl-2-docosahexaenoyl (or eicosapentaenoyl) Glycerol, JAOCS, vol. 78, No. 3, pp. 285-289 (Mar. 2001).
U. Schmid et al., Optimization of the Reaction Conditions in the Lipase-Catalyzed Synthesis of Structured Triglycerides, JAOCS, vol. 75, No. 11, pp. 1527-1531 (1998).
R. Irimescu et al., Enzymatic Synthesis of 1,3-Dicapryloyl-2-eicosapentaenoylglycerol, JAOCS, vol. 77, No. 5, pp. 501-506 (May 2000).
H. Breivik et al., Preparation of Highly Purified Concentrates of Eicosapentaenoic Acid and Docosahexanoic Acid, JAOCS, vol. 74, No. 11, pp. 1425-1429 (1997).

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