Process for the production of diacylglycerol

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase

Reexamination Certificate

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C435S159000

Reexamination Certificate

active

07943335

ABSTRACT:
The present invention provides a process for producing a diacylglycerol, which comprises, reacting triacylglycerol with water and an enzyme preparation to obtain a mixture comprising of diacylglycerol, monoacylglycerol and free fatty acid; removing water content in the mixture by way of dehydration; and separating monoacylglycerol, free fatty acid and residual triacylglycerol by at least one separation method to obtain a high-purity diacylglycerol. An oil or fat composition comprising of diacylglycerol obtained from the process and phytosteryl esters and/or ferulic acid esters in an amount of from 0.5% to 25% by weight of diacylglycerol is also provided.

REFERENCES:
patent: 4678580 (1987-07-01), Brady et al.
patent: 5149642 (1992-09-01), Mazur et al.
patent: 6139897 (2000-10-01), Goto et al.
patent: 6261812 (2001-07-01), Yamada et al.
patent: 6361980 (2002-03-01), Sugiura et al.
patent: 2003/0054082 (2003-03-01), Koike et al.
International Search Report for PCT/MY2006/000034, mailed on May 8, 2007.
Written Opinion of the International Searching Authority for PCT/MY2006/000034, mailed on May 8, 2007.

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