Process for the production of cellulolytic and...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound

Reexamination Certificate

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C435S069100, C435S099000, C435S161000, C435S174000, C435S209000, C435S254100

Reexamination Certificate

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07494792

ABSTRACT:
A process for producing cellulolytic and/or hemicellulolytic enzymes uses the residue from the ethanolic fermentation of enzymatic hydrolyzates of cellulosic or ligno-cellulosic materials. This process may be integrated into a process for the production of ethanol from cellulosic or ligno-cellulosic materials which comprises the following steps:1) chemical and/or physical pre-treatment of a cellulosic or ligno-cellulosic substrate;2) enzymatic hydrolysis of the pre-treated substrate using cellulolytic and/or hemicellulolytic enzymes;3) ethanolic fermentation, by a suitable alcohologenic microorganism, of the hydrolyzate from step (2) and production of a fermentation must; and4) separation of the alcohologenic microorganism used in step (3), separation/purification of the ethanol and production of an aqueous phase constituting a residue;and in which said residue serves for the production of the cellulolytic and/or hemicellulolytic enzymes used in step 2).

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patent: WO 03052055 (2003-06-01), None
Chahal Parminder S et al: “Production of cellulose in solid-state fermentation with Trichoderma reesei MCG 80 on wheat straw” Appliced Biochemistry and Biotechnology, vol. 57*58, No. 0, 1996, pp. 433-442, XP008054568.
Warzywoda M et al: “Production and Characterization of Cellulolytic Enzymes From Trichoderma-Reesei Grown on Various Carbon Sources” Bioresource Technology, vol. 39, No. 2, 1992, pp. 125-130, XP008054567.

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