Process for the production of an insulin-producing cell line of

Details Process for the production of an insulin-producing cell line of Process for the production of an insulin-producing cell line of

1980-06-13

1982-06-01

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

435235, 435240, 435241, 435172, 435948, C12N 502, C12P 2100, C12N 1500

Patent

active

043328938

ABSTRACT:
A process for producing an insulin-producing conditionally transformed pancreatic cell line is provided. The process of the present invention comprises the steps of preparing and culturing a population of insulin-producing pancreatic beta cells, preparing and culturing a suitable strain of Rous sarcoma virus containing a temperature sensitive lesion in the viral transforming or sarc gene, and infecting the insulin-producing pancreatic cells with the temperature sensitive virus. The infected pancreatic cells are conditionally transformed by the temperature-sensitive virus to provide an insulin-producing system especially suited for both in vitro and in vivo application. Methods for evaluating the safety of the system for application in vivo are also disclosed.

REFERENCES:
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