Process for the production of a virus-free concentrate of thromb

Details Process for the production of a virus-free concentrate of thromb Process for the production of a virus-free concentrate of thromb

1995-06-05

1998-03-03

Sayala, Chhaya D.

Drug, bio-affecting and body treating compositions

Enzyme or coenzyme containing

Hydrolases

424529, 424530, 530830, 438214, A61K 3848

Patent

active

057231230

ABSTRACT:
The invention relates to a process for producing a virus free preparation of thrombin, and the use of said virus free concentrate as a pharmaceutical. For example, the invention relates to a process for the production of a purified and virus free preparation of thrombin which comprises adding a soluble salt in a concentration of from 0.5 mol/l up to the saturation limit thereof, said soluble salt having an anion that forms a sparingly-soluble salt or a soluble complex with calcium, to a solution of a prothrombin complex which has been purified on an anion exchanger and subjected to virus inactivation, said solution having a catalytic amount of thrombin present as a result of the purification, the viral inactivation, or added thereafter at a concentration of greater than zero up to 200 units of thrombin per ml of solution.

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