Process for the production of a protein

Details Process for the production of a protein Process for the production of a protein

1989-08-10

1990-12-11

Draper, Garnette

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Separation or purification

530413, 530415, 530416, 530417, 530820, 530408, 530409, 530410, 435 691, 435 697, C07K 320

Patent

active

049772481

ABSTRACT:
A method for the renaturation of unfolded proteins comprises reversibly immobilizing the denatured protein on a solid phase and inducing folding of the immobilized protein by progressively reducing with time the concentration of a denaturing agent in the solvent in contact with the solid phase. The refolded protein is recovered from the solid phase in native form. The proteins can be folded and recovered in high yield in a small volume of buffer.

REFERENCES:
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patent: 4572798 (1986-02-01), Koths et al.
patent: 4656255 (1987-04-01), Seely
patent: 4659568 (1987-04-01), Heilman
patent: 4766205 (1988-08-01), Rausch
patent: 4766224 (1988-08-01), Ghosh-Dastidar
patent: 4839419 (1989-06-01), Kraemer et al.
Orsini et al, JBC 253, 1978, pp. 3453-3458.
Odorzynski et al., JBC 254, 1979, pp. 4291-4295.
Light, Bio Techniques, vol. 3(4), 1985, pp. 298-306.
Sofer et al, Bio Techniques, 1983 (Nov./Dec.), pp. 198-203.

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