Process for the production of 7-ADCA via expandase activity on p

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound having a 1-thia-5-aza-bicyclo

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435 46, 435 51, 4352545, 540218, C12P 3702, C12P 3502, C07D50100

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057311650

ABSTRACT:
An overall process for the preparation and recovery of 7-aminodesacetoxycephalosporanic acid (7-ADCA) via enzymatic ring expansion activity on penicillin G, using a Penicillium chrysogenum transformant strain expressing expandase.

REFERENCES:
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Cantwell et al., "Cloning and expression of a hybrid Streptomyces clavuligerus ce.function.E gene in Penicillium chrysogenum," Curr Genet 17:213-221 (1990).
Yeh et al., "Biochemical characterization and Evolutionary Implication of .beta.-Lactam Expandase/Hydroxylase, Expandase and Hydroxylase" 50 Years of Penicillin Application (H. Kleinkauf and H. Von Dohren, Editors) pp. 209-210 (1991).
Aharonowitz, Y., et al. (1992) Ann. Rev. Microbiol. 46:461-495.
Baldwin, et al. (1988) Natural Product Reports 5(2):129-145.
Cantwell, et al. (1992) Proc. R. Soc. Lond. B. 248:283-289.
Coque, et al. (1993) Mol. Gen. Genet. 236:453-458.
Cortes, et al. (1987) J. Gen. Microbiol. 133:3166-3174.
Crawford, et al. (1995) Bio/Technology 13:58-61.
Ingolia, et al. (1989) Med. Res. Rev. 9:245-264.
Maeda, et al. (1995) Enzyme and Microbial Technology 17:231-234.

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