Process for the production, desiccation and germination of conif

Chemistry: molecular biology and microbiology – Spore forming or isolating process

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43524045, 43524054, 47 576, 47DIG9, C12N 500, A01C 106

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active

051837579

ABSTRACT:
A process to assist in germination of spruce somatic embryos. The embryos are desiccated at humidity, preferably in the range of 85 to 99%. A process that differentiates somatic embryos of conifer comprises contacting embryogenic calli with a medium containing abscisic acid. A process for determining the quality of a plant to embryo is also described. That process comprises identifying the storage protein content of the embryo and comparing that content with the storage protein content of mature embryos of the same species.

REFERENCES:
Hakman et al. 1988, Physiol. Plant. 72(3): 579-587.
Becwar et al. 1987, TAPPI J 70(4): 155-160.
Hammatt et al. 1987. J. Plant Physiol. 128(3): 219-226.
Boulay et al. 1988. Plant Cell Reports 7: 134-137.
Bewley, J. D. and Black, M. (1985) "Maturation Drying, The Effects of Water Loss on Development in Seeds"; Physiology of Plant Development, Chapter 2.4; Plenum Press N.Y.; pp. 70-73.
Becwar, M. R. et al; "Maturation, Germination, and Conversion of Norway Spruce Somatic Embryos to Plants" (1989), In Vitro Cell. & Dev. Biol. 25: 575-580.

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