Process for the production and use of a stable 6-phosphogluconol

Details Process for the production and use of a stable 6-phosphogluconol Process for the production and use of a stable 6-phosphogluconol

1993-02-25

1995-06-20

Wityshyn, Michael G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435 15, 435 17, 435814, C12Q 134, C12Q 148, C12Q 150

Patent

active

054260345

ABSTRACT:
The invention concerns a new 6-phosphogluconolactonase which has an enzyme activity of at least 90% with respect to the initial activity after about 20 hours at pH 7.0 and 20.degree. C. In addition a process is described for the production of the enzyme from Leuconostoc. The enzyme is preferably suitable for the determination of ATP or reaction partners which can be converted into 6-phosphogluconolactone.

REFERENCES:
patent: 3907644 (1975-09-01), Mollering et al.
patent: 4740488 (1988-04-01), Rondo
Bauer, H. P. 6-phosphogluconolactonase Eur J Biochem 133, 163-168 (1983).
Medina-Puerta M. M. Purification of 6-PGL Biochem Int 1988 17(6) 1011-1019.
Beutler E. et al., Brit. Journal of Haemat., vol. 62/No. 3 (1986), 557-586.
Beutler, E. and Kuhl W., J. Lab. Clin. Med., vol 107/6 (1986), 502-507.
Gerhardt, Methods of Enzymatic Analysis, Third Edition, vol. III, "Enzymes 1: Oxidoreducases, Transferases", pp. 510-539, 1983.

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