Process for the production and renaturation of recombinant, biol

Details Process for the production and renaturation of recombinant, biol Process for the production and renaturation of recombinant, biol

1993-07-30

1995-07-18

Walsh, Stephen G.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435 691, 4353201, 530412, C12N 912, C12N 1554, C12N 1570, C07K 136

Patent

active

054340675

ABSTRACT:
In a process for the production of recombinant, biologically active, eukaryotic alkaline phosphatase a DNA sequence coding for a eukaryotic alkaline phosphatase is expressed in a prokaryotic host cell and the expression product is obtained in an active form by cell lysis, solubilization under denaturing conditions and subsequent renaturation. In this process the renaturation step is carried out in the presence of one or several stabilizing agents.

REFERENCES:
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Low et al., Biochem. J. 241:615-619 (1987).
Bingham et al., "Purification and Properties of Alkaline Phosphatase . . . " J. Dairy Sci. 75: 3394-3401 (Dec. 1992).
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Holyaerts et al., Biochem. J. 286:23-30 (Aug. 1992).
Hermann, R., EPO Applied Technology Series, vol. 12: Protein Folding EPO, The Hauge, The Netherlands (1993) pp. 1, 44-45, 62-65, 108-110.
Torchilin, V. P., Enzyme Microb. Technol. vol. 1, pp. 74-81 (1979).
Cleland, J. L., et al. Biotechnology, vol. 8, pp. 1274-1278 (1990).
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Chemical Abstract, Enzymes vol. 69, p. 3871, abstract No. 41426m (1968).
Chemical Abstract, vol. 91, p. 624 abstract No. 89776g (1979).

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