Process for the production and extraction of thermostable supero

Details Process for the production and extraction of thermostable supero Process for the production and extraction of thermostable supero

1994-06-09

1996-07-16

Lilling, Herbert J.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

4352571, 435814, C12N 902

Patent

active

055366546

ABSTRACT:
Process for the production and extraction of thermostable super-oxide-dismutases from a photosynthetic microorganism cell. The thermostable superoxide-dismutase production and extraction process consists a) of culturing in the temperature range 40.degree. to 80.degree. C., in a closed photoreactor made from a light-transparent material and which is thermally resistant within said range, aerobic, photosynthetic, thermophilic microorganisms, which produce oxygen and grow exponentially in said range, said microorganisms being suspended in a culture medium and chosen from among microalgae and cyanobacteria and b) extracting from the culture medium the freshly produced, thermostable superoxide-dismutases, by cellular crushing, ultrafiltration and selective precipitation.

REFERENCES:
patent: 4868123 (1989-09-01), Berson et al.
patent: 5179012 (1993-01-01), Gudin et al.
Derwent Abs. J06069181 (Mar. 1990) Idemitsu Petrochem KK.
Patent Abstracts of Japan, vol. 014, No. 250 (C-0723) 29 May 1990 & JP-A-02 069 181 (Tadashi Matsunaga) 8 Mar. 1990.
Health Physics, vol. 53, No. 3, Sep. 1987, New York, pp. 281-286, Conter A. et al., "Radiation Stimulation During the Early Stationary Growth Phase in Synechococcus Lividus and its Correlation with Photooxidative Stress Occurring before the Stationary Phase".
Biochim. Biophys. Acta, vol. 438, No. 2, 8 Jul. 1976, pp. 380-392, Lumsden et al., "Purification and Physicochemical Properties of Superoxide Dismutase from Two Photosynthetic Microorganisms".

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