Process for the preservation of ram semen by freezing

Details Process for the preservation of ram semen by freezing Process for the preservation of ram semen by freezing

1974-12-16

1976-08-03

Rosen, Sam

Drug, bio-affecting and body treating compositions

Fermentate of unknown chemical structure

Having a known elemental analysis

195 18, A61K 3552, C12K 900

Patent

active

039730034

ABSTRACT:
The process according to the invention consists in that the freshly collected ram semen is first diluted at between 25.degree. and 32.degree.C and notably at 30.degree.C, in a diluent without glycerol containing lactose and egg yolk, and in then diluting, at between 3.degree. and 5.degree.C and notably at 4.degree.C, the mixture previously obtained in a diluent containing glycerol, said diluent essentially comprising powdered milk and sodium citrate, in conditioning in straws the so diluted semen and in freezing it in liquid nitrogen at about -75.degree.C. The invention also relates to the diluent used in the above process.
The process is useful for preserving ram semen by freezing for subsequent artificial insemination of ewes.

REFERENCES:
patent: 3185623 (1965-05-01), Smith et al.
patent: 3431172 (1969-03-01), Rajamannan

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