Process for the preparation of the highly pure enzyme kallikrein

Details Process for the preparation of the highly pure enzyme kallikrein Process for the preparation of the highly pure enzyme kallikrein

1982-01-12

1983-07-12

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435815, C12N 964

Patent

active

043931405

ABSTRACT:
The invention relates to a process for the preparation of the highly pure enzyme kallikrein from organ extracts, in particular from swine pancreas extracts, the accompanying proteolytic activity of the swine pancreas extract being separated off by chromatography on ion exchangers and the highly pure enzyme kallikrein being obtained by chromatography on support materials with a kallikrein-trypsin inhibitor (basic pancreatic trypsin inhibitor=BPTI) covalently bonded in a particular manner.

REFERENCES:
patent: 3100736 (1963-08-01), Werle et al.
patent: 3660237 (1972-05-01), Schultz
patent: 3905870 (1975-09-01), Kutzbach et al.
patent: 4038141 (1977-07-01), Tokuyasu et al.
patent: 4252902 (1981-02-01), Fuji et al.
Methods in Enzymology, vol. 45, pp. 289-303, (1976).

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