Process for the preparation of riboflavin

Details Process for the preparation of riboflavin Process for the preparation of riboflavin

1984-08-21

1988-12-27

Warren, Charles F.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound other than saccharide containing...

435 85, 435255, 435256, 435119, C12P 2500, C12N 116

Patent

active

047940816

ABSTRACT:
Riboflavin is obtained directly from the culture in a high yield by cultivating a riboflavin-producing microorganism in a medium using a lower (C.sub.1 to C.sub.4) aliphatic compound, separating riboflavin contained in the culture in the form of heated aqueous solution from solid matters, and crystallizing riboflavin from the heated aqueous solution.
Riboflavin is also prepared in a high yield by cultivating a riboflavin-producing yeast belonging to the genus Saccharomyces which has purine requirements and/or resistance to 3-amino-1,2,4-triazole in a medium and collecting riboflavin formed and accumulated in the medium.
Moreover, riboflavin is prepared in a high yield even in the presence of iron ions by preliminarily cultivating in liquid a riboflavin-producing yeast belonging to the genus Saccharomyces and then cultivating it in a riboflavin-producing medium containing zinc ions.

REFERENCES:
patent: 4118512 (1978-10-01), Eichelburg
patent: 4310635 (1982-01-01), Hasegawa et al.
Giri et al., "Studies on the Synthesis of Riboflavin by a Mutant Yeast, Saccharomyces cerevisiae" J. Bact., vol. 67 (1954) pp. 309-313.
Gross et al., "Identification of Mutants Defective in the First and Second Steps of De Novo Protein Synthesis in S. cerevisiae" Biochimica et Biophysica Acta 247(1971) pp. 13-21.
Bacher et al., "The Structure of Purine Precursor in Riboflavin Biosynthesis" Angew. Chem. Internat. Edit., vol. 8 (1969) No. 5, pp. 371-372.
Hilton, J. L., "Inhibitions of Growth and Metabolism by 3-Aminotriazole (Amitrole)" J. Agr. Food Chem. 17(2) Mar.-Apr. 1969.
Klopotowski, T. "Adaptation of Yeast to 3-Amino-1,2,4-Triazole" Acta Biochim. Polon., 10:199-208, 1963.
Klopotowski et al., "Partial Reversal by Purine and Pyrimidine Bases of Yeast Growth Inhibition Produced by 3-Amino-1,2,4-Triazole" Acta Biochim. Polon., 13: 153-163 (1966).
Hilton, J. L., "Modes of Action of 3-Amino-1,2,4-Triazole: Current Status" Isotope Weed Research Proc. Symp. Vienna (1965) pp. 71-83.
Riboflavin Biosynthesis by Candida robusta, Parts 1 and 2; by Shoichi Takao, Agr. Biol. Chem., vol. 28, No. 8, pp. 559-565, 1964.
Nutritional Growth Requirements for Butyribacterium methylotrophicum on Single Carbon Substrates and Glucose by Thomas T. Moench and J. G. Zeikus; Current Microbiology, vol. 9, pp. 151-154, 1983.

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