Process for the preparation of purple membrane containing bacter

Details Process for the preparation of purple membrane containing bacter Process for the preparation of purple membrane containing bacter

1990-04-17

1992-01-07

Moezie, F. T.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435711, 530412, 530417, 530802, 530825, 530400, C07K 320, C07K 1522

Patent

active

050791494

ABSTRACT:
The invention relates to a process for the preparation of purple membrane containing bacteriorhodopsin, which process comprises obtaining, in a manner know per se, the cell membrane from halobacteria cells, and subjecting the material to gel filtration chromatography in order to isolate the purple membrane from the cell membrane.

REFERENCES:
Schegle et al., EMBO J. vol. 7(9), pp. 2925-34 (1988).
Seignevret et al., FEBS Lett. vol. 228 (1) pp. 79-84 (1988).
Hegemann et al. EMBO J. vol. 1 (10) pp. 1177-84 (1982).
Huang et al., Proc. Natl. Acad. Sci. U.S.A. vol. 77 (1), pp. 323-7 (1980).
Happe et al., Biochem. Biophys. Res. Comm. vol 72 (4) pp. 1504-11 (1976).
Biosystems 1986,19, 223-236, The Bacteriorhodopsin Model Membrane System as a Prototype Molecular Computing Element, F. T. Hong.
Biophysics 1985, 30 (5), 962-967, Biological Light-Sensitive Complexes as Technical Information Photocarriers, N. N. Vsevolodov, G. R. Ivanitskii.
Nature 1971, 233, 149-154, Rhodopsin-like Protein from the Purple Membrane of Halobacterium Halobium D. Oesterhelt, W. Stoeckenius.
Methods in Enzymology 1974, 31, 667-678, Isolation of the Cell Membrane of Halobact. Halobium and its Fractionation into Red and Purple Membrane, D. Oesterhelt, W. Stoeckenius.
Preparative Biochemistry 1975, 5 (2), 161-178, Improved Isolation Procedures for the Purple Membrane of Halobacterium Halobium. B. M. Becher, J. Y. Cassim.

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