Process for the preparation of polypeptides utilizing a charged

Details Process for the preparation of polypeptides utilizing a charged Process for the preparation of polypeptides utilizing a charged

1983-03-03

1985-07-30

Marantz, Sidney

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

435 70, 435 71, 435803, 4351723, 935 47, 935 51, 2601125R, C12P 2100, C12P 2102, C12P 2104, C12N 1500

Patent

active

045322074

ABSTRACT:
A method for the production of polypeptides utilizing a polymer of charged amino acids and an exopeptidase. A DNA sequence coding for the charged amino acid polymer is inserted adjacent to one end of the polypeptide gene. The polypeptide-charged amino acid polymer fusion product is removed from most contaminants by selecting for the charged amino acid polymer during a first isolation step. Next the charged amino acid polymer is hydrolyzed using an exopeptidase which leaves the polypeptide active. A second isolation step removes the polypeptide from any remaining contaminents. An example using polyarginine and carboxypeptidase B to produce urogastrone is included.

REFERENCES:
patent: 4237224 (1980-12-01), Cohen et al.
patent: 4336336 (1982-06-01), Silhavy et al.
patent: 4366246 (1982-12-01), Riggs
patent: 4375514 (1983-03-01), Siewert et al.
patent: 4399216 (1983-08-01), Axel et al.
patent: 4411994 (1983-10-01), Gilbert et al.
Lehninger, Biochemistry, Worth Publishers, New York, 1975, pp. 157-172 and 560.
Bolivar et al., "Plasmids of Escherichin Coli as Cloning Vectors", in Methods in Enzymology: Recombinant DNA, vol. 68, Wu Ed., (New York, Academic Press, 1979) pp. 261-262.
Livingston, "Immunoaffinity Chromatography of Proteins", in Methods in Enzymology: Affinity Techniques, vol. 34, Jakoby et al. Ed., (New York, Academic Press, 1974), pp. 723-731.
Urdea et al., Chemical Abstracts, vol. 100, 1984, Abstract No. 115726s, "Chemical Synthesis of a Gene for Human Epidermal Growth Factor Urogastrone and its Expression in Yeast".
Sassenfeld et al., Chemical Abstracts, vol. 100, 1984, Abstract No. 99285q, "A Polypeptide Fusion Designed for the Purification of Recombinant Proteins".
Chemical Abstracts, vol. 93, 1980, Abstract No. 37842x, Hollenberg et al.
Gregory, H., et al., Int. J. Peptide Protein Res., 9, 107-118, (1977).
Tacon, W., Carey, N. and Emtage, S. Molec. Gen. Genet., 177, 427-438, (1980).
Smith, J., et al., Nucleic Acids Res., 10, 4467-4482 (1982).
Handbook on Affinity Chromatography, Pharmacia Fine Chemicals, Box 175, S-751-04, Uppsala, Sweden.
Ornstein, L. and Davis, B. J., Ann. N.Y. Acad. Aci., 121, 321-427, (1964).
Laemmli, U. K., Nature (London), 277, 680 (1970).
Corbin, M. C., Hugli, T. E. and Muller-Eberhard, H. J., Anal. Biochem., 73, 41-51, (1976).
Imai, K., Bohlen, P., Stein, S. and Udenfriend, D., Arch. Bioch. Biophys., 161, 161-163, (1974).
Eisen, H. N., Immunology, Harper and Row, USA, 395-396, (1974).
Davis, A. R., et al., P.N.A.S. USA, 78, No. 9, 5376-5380, (1981).
Goeddel, D. V., et al., P.N.A.S. USA, 76, 106-110 (1979).

Affiliated with

Also associated with

Rating

Process for the preparation of polypeptides utilizing a charged does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Process for the preparation of polypeptides utilizing a charged , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for the preparation of polypeptides utilizing a charged will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2373006