Process for the preparation of N-benzyloxycarbonyl-alpha-L-aspar

Details Process for the preparation of N-benzyloxycarbonyl-alpha-L-aspar Process for the preparation of N-benzyloxycarbonyl-alpha-L-aspar

1991-07-10

1994-01-18

Knode, Marian

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

435108, 435109, 435129, C12P 2102

Patent

active

052799468

ABSTRACT:
Disclosed is a process for the preparation of N-benzyloxycarbonyl-.alpha.-L-aspartyl-L-phenylalanine methyl ester wherein a protease is used to catalyze the condensation reaction of N-benzyloxycarbonyl-L-aspartic acid with L-phenylalanine methyl ester in a system consisting of ethyl acetate, citrate buffer with the addition of glycerol as a dispersing agent.

REFERENCES:
patent: 4116768 (1978-09-01), Isowa et al.
patent: 4935355 (1990-06-01), Ulmer et al.
Nakanishi et al., "Continuous Synthesis of N-(Benzylcrycarbonyl)-L-Aspartyl-L-Phenylalanine Methyl Ester with Immobilized Thermolysin in an Organic Solvent," Biotechnology, vol. 3, pp. 459-464, 1985.
Nakanishi et al., "Kinetics and Equilibruim of Enzymatic Synthesis of Peptides in Aqueous Organic Biphanic Systems," Eur. J. Biochem. vol. 161, pp. 541-549 (1986).
Nakamura et al, "N . . . Production by Immobilized Nocardia opaca: Thermolysin under Hydrogen High Pressure in a Water Organic Solvent System," J. Ferment. Bioenginer vol. 67, No. 6 pp. 399-403 1989.
Toi et al., "Papain Catalyzed Esterfication in Polar, Organic Solvents," Biotech Letters, vol. 11, No. 3 pp. 173-176 1989.

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