Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process...
Reexamination Certificate
2001-11-02
2004-03-09
Meller, Michael (Department: 1651)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
C435S198000
Reexamination Certificate
active
06703218
ABSTRACT:
FIELD OF THE INVENTION
The present invention relates to a process for the preparation of linalool oxide or of linalool oxide-containing mixtures.
BACKGROUND OF THE INVENTION
Linalool oxide is an aroma material, which has been known for a long time and is used in a large number of aromas. Linalool oxide, moreover, occurs both in the furanoid form as a nine-membered ring (2-methyl-2-vinyl-5-(&agr;-hydroxyisopropyl)tetrahydrofuran, CAS 60047-17-8; cis and trans: CAS 5989-33-3 and 34995-77-2), and in the pyranoid form as 6-membered ring (2,2,6-trimethyl-6-vinyltetrahydro-2H-pyran-3-ol, CAS 14049-11-7; cis and trans: CAS 14009-71-3 and 39028-58-5). It occurs naturally in essential oils such as rosewood oil and osmanthus abs. and fruits (Volatile Compounds in Food, Qualitative and Quantitative Data, 7th edition, 1996, TNO Nutrition and Food Research Institute, Zeist, the Netherlands) and has as a flowery-earthy, slightly bergamot-like odor (Bauer, Garbe, Surburg, Common Fragrance and Flavor Materials, second edition, VCH, Weinheim 1990, p. 114). The flavor impression of linalool oxide is in the citrus, apricot, blueberry, flowery direction.
The person skilled in the aroma sector distinguishes three areas: natural, nature-identical and synthetic aromas. Synthetic aromas are of only minor interest because consumer acceptance is not particularly high. One example of the preparation of a nature-identical aroma material is described in JP A 59225176. This publication relates to the production of linalool oxide. In this case, unnatural starting materials are converted by chemical synthesis using strong mineral acids into linalool oxide. Such a product can be categorized only as “nature-identical”, but not as natural, and must, therefore, not be used to produce natural aromas.
Natural aromas, and thus also the natural aroma materials present therein, are to be categorized as having the highest value. This is also shown clearly by the considerably higher prices, which can be obtained for natural aromas. In addition, consumer interest in natural products and thus, also in natural aromas required for their production is increasing.
It is therefore an aim of the aroma industry to have natural building blocks for natural aromas available in sufficient quantity and quality. However, it is usually not economical to isolate these individual building blocks from natural extracts.
One example of the production of linalool oxide by fermentation is disclosed by
Demyttenaere
(Demyttenaere, J. C. R., 1998, Biotransformation of terpenes by fungi for the production of bioflavors, Meded.—Fac. Landbouwkd. Toegepaste Biol. Wet., 63 (4a), 1321-1324, and Demyttenaere, J. C. R.; Willemen, H. M., 1998, Biotransformation of linalool to furanoid and pyranoid linalool oxides by
Aspergillus niger
, Phytochemistry, 47 (6),1029-1036). The biotransformation of terpene alcohols with the aid of
Aspergillus niger
and Penicillium strains is described therein. However, this process is very complicated to carry out, the conversion is very slow, and the yields of linalool oxide are only 20-33%.
In addition, the use of enzymes for epoxidation of double bonds in fatty acids and alkenes is known (1. PCT WO 91/04333. 2. Jarvie, A. W. P.; Overton, N.; St. Pourcain, C. B., 1998, Enzymatic epoxidation of polybutadiene, Chem. Comm., 177-178. 3. Lemoult, S. C.; Richardson, P. F.; Roberts, S. M., 1995, Lipase catalyzed Baeyer-Villiger Reactions, J. Chem. Soc. Perkin Trans. I, 89-91. 4. Rüsch, M.; Warwel, S., 1996, Peroxy fatty acids: lipase catalyzed preparation and epoxidation, Lipid Technology, 7, 77-80. 5. Miurra, Y.; Yamane, T., 1997, Epoxidation of alkanes and cycloalkanes by microbial lipases immobilized with photo crosslinkable resin prepolymer, Biotechnol. Lett., 19, 7, 611-613). This involves epoxidation of double bonds analogous to the chemical reaction with a peroxycarboxylic acid. In these cases, the enzyme lipase (E. C. 3.1.1.3, CAS No. 9001-62-1, for example from
Pseudomonas cepacia
, DSM 3959
, Candida antarctica
DSM 3855, DSM 3908, DSM 3909
Mucor mieh, Humicola lanuginosa
, DSM 3819, DSM 4109
, Humicola brevispora
, DSM 4110
, Humicola brevis
var.
thermoidea
, DSM 4111
, Humicola isolens
, DSM 1800) catalyzes, in the presence of hydrogen peroxide, intermediate formation of the peroxycarboxylic acid (WO 91/04333).
Numerous publications disclose the use of lipases on the laboratory or manufacturing scale, essentially for esterifications or transesterifications. However, none of these descriptions describes the preparation of furanoid or pyranoid linalool oxide by an enzymatic route.
SUMMARY OF THE PRESENT INVENTION
It is an object of the present invention now to produce linalool oxide or a linalool oxide-containing mixture, which can be categorized as natural and can be used to produce natural aromas. An additional aim is for the yields to be distinctly higher than in the state of the art to date. A particular aim is to achieve yields above 90%.
A process for the preparation of linalool oxide or of linalool oxide-containing mixtures has been found and is characterized in that linalool or linalool-containing mixtures are oxidized in the presence of an enzyme.
DETAILED DESCRIPTION OF THE INVENTION
It is possible to use in the process according to the present invention both D-linalool and L-linalool. These substances may be in pure form or in the form of a mixture. It is likewise possible to employ the linalools mixed with other substances.
Suitable other substances are essential oils, absolutes or extracts. In a preferred embodiment, linalool can be present in the essential oils such as rosewood oil, coriander oil, basil oil, lavender oil etc. It is also possible in these cases for D-linalool or L-linalool to be present as single substances in the reaction medium. A mixture of D-linalool and L-linalool in the reaction medium is, of course, likewise suitable for the purposes according to the present invention.
The enzymes employed according to the present invention are preferably hydrolases, specifically lipases or proteases. Lipases are suitable and particularly preferred according to the present invention. In particular, a mobilized lipase is preferred, for example Chirazym C2 L2 from Roche Diagnostics, Penzberg, Germany, or Novozym 435 from Novo Nordisk, Denmark.
The enzymes are preferably those which can be obtained with the aid of the following microorganisms:
Pseudomonas cepacia
, for example DSM 3959
, Candida antarctica
, for example DSM 3855, DSM 3908, DSM 3909
Mucor mieh, Humicola lanuginosa
, for example DSM 3819, DSM 4109
, Humicola brevispora
, for example DSM 4110
, Humicola brevis var. thermoidea
, for example DSM 4111
, Humicola isolens
, for example DSM 1800.
Yeasts are preferred as enzyme producers. Candida species in the form of pure cultures or mixed cultures of these yeasts are more preferred for this purpose, especially the
Candida antarctica
. Accordingly, lipases from
Candida antarctica
are most preferred for the process according to the present invention.
In the process according to the present invention, the oxidation of linalool was preferably carried out in the presence of said enzymes and of an oxidizing agent. Hydrogen peroxide is preferred. The oxidizing agent can be added before or during the oxidation process. It is likewise possible according to the present invention for the medium to have such a composition and/or for the reaction conditions to be chosen such that the required oxidizing agent is formed during the progress of the process according to the present invention. In particular, the formation of oxidizing agent can be carried out with the use of enzymes. Examples thereof, are oxidases, for example, glucose oxidase. It is also possible to use as non-enzymatic catalysts for preparing the hydrogen peroxide.
It is, thus, possible in the variant according to the present invention, for example, to choose the combination of the medium and of the enzymes such that the hydrogen peroxide is produced in situ during the process. This may involve the hydrogen peroxide production be
Gatfield Ian-Lucas
Hilmer Jens-Michael
Haarman & Reimer GmbH
Meller Michael
Pendorf & Cutliff
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