Process for the preparation of Limulina lysate

Details Process for the preparation of Limulina lysate Process for the preparation of Limulina lysate

1973-12-10

1976-05-04

Padgett, Benjamin R.

Compositions

Nonreactive analytical, testing, or indicating compositions

23230B, 195 99, 1951035R, 424 2, 424 12, 424 95, 424101, 424253, C09K 300, G01N 3100, G01N 3316, C12K 104, A61K 3556, A61K 3152

Patent

active

039546632

ABSTRACT:
A process comprising extracting the blood of horseshoe crabs (Limulina) into an isotonic buffer solution containing an agent which inhibits the agglutination of amebocyte, separating the amebocyte from said solution, breaking the amebocyte, and recovering Limulina lysate, which is characterized in that methyl derivatives of xanthine such as theophyline, theobromine, or caffeine, or their salts, are used as the amebocyte's agglutination-inhibiting agent is disclosed.

REFERENCES:
patent: 3729947 (1973-05-01), Higuchi
patent: 3740433 (1973-06-01), Clody et al.
Thye Yin, E., et al.; Biochem. Biophys. Acta., Vol. 261, p. 284-289 (Jan. 1972).
Bryan, F. T.; et al.; Science, Vol. 144, p 1147-1148 (May 1964).
Chemical Abstracts, Vol. 78, 95202q (Jan.-June 1973).
Chemical Abstracts, Vol. 76, 70459z (Jan.-June 1972).
Chemical Abstracts, Vol. 69, 9114x (1968).
Chemical Abstracts, Vol. 79, 28578z (June-Dec. 1973).
Hochstein, Donald H., et al., Bulletin of the Parenteral Drug Association, Vol. 27, No. 3, pp. 139-148 (6/73).
Solum, N. O.; Thrombosis Et Diathesis Haemorrhagica, Vol. 23, pp. 170-181 (1970).

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