Process for the preparation of human leukocyte and human gamma i

Details Process for the preparation of human leukocyte and human gamma i Process for the preparation of human leukocyte and human gamma i

1984-12-10

1987-09-29

Hazel, Blondel

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

424 85, 435811, 530351, C12P 2100, A61K 4502

Patent

active

046968997

ABSTRACT:
The invention relates to a process for the preparation of .alpha.- and .gamma.-interferon by isolating the buffy coat fraction of blood, removing the erythrocytes, suspending the leukocytes in a suitable nutrient medium and treating with an .alpha.-interferon inducer and with a .gamma.-interferon inducer, which comprises producing .alpha.-interferon and .gamma.-interferon one after the other in the same leukocyte culture by pre-treating the suspension obtained after the separation of the leukocyte/buffy coat/fraction of blood, removal of the erythrocytes and suspending the leukocytes in a suitable nutrient medium with - or .beta.-interferon, contacting with an .alpha.-interferon inducer, separating the liquid containing the .alpha.-interferon from the cells, if desired recovering the -interferon, washing and suspending the cells in a suitable nutrient medium, treating with a mitogenic agent, separating the liquid comprising .alpha.-interferon from the cells and if desired recovering .alpha.-interferon and if desired making .alpha.-interferon free of .alpha.-interferon.
The advantage of the process is that .alpha.- and .gamma.-interferon are produced from the same leukocyte culture in two steps. This makes the process more economical and enables an effective utilization of the limitedly available leukocytes.

REFERENCES:
patent: 4296025 (1981-10-01), Sugimoto
Wiranowska-Stewart, J. Interferon Research, vol. 1, pp. 315-321, 1981.
Cantell et al., Proc. of Tissue Culture Association Workshop, Lake Placid, New York, pp. 35-38, 1973.
Torna et al., J. Biol. Chem., vol. 251, pp. 4810-4816, 1976.

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