Process for the preparation of cholesterol oxidase

Details Process for the preparation of cholesterol oxidase Process for the preparation of cholesterol oxidase

1975-11-20

1977-02-15

Shapiro, Lionel M.

Textiles: fiber preparation

C12D 1310

Patent

active

040081273

ABSTRACT:
Cholesterol oxidase which oxidizes cholesterol with oxygen to give cholestenone and hydrogen peroxide, is prepared by culturing Nocardia erthropolis ATCC 17895, Nocardia erthropolis ATCC 4277, Nocardia formica ATCC 14811 or Proactinomyce erythropolis NCIB 9158 first on a peptone-containing mineral salt medium and thereafter on at least one sterol compound as the sole source of carbon, and then isolating the cholesterol oxidase formed from the cultured micro-organism, wherein said sterol compound is of the formula ##STR1## in which R.sub.1 and R.sub.2 are hydrogen or together represent a double bond,

REFERENCES:
patent: 3907642 (1975-09-01), Richmond
patent: 3909359 (1975-09-01), Goodhue et al.

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