Process for the preparation of an improved Brucella strain...

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Reexamination Certificate

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C536S023700, C536S024100, C536S024200, C536S024300, C536S024320, C424S009100, C424S009200, C424S093100, C424S093200, C424S093400

Reexamination Certificate

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07541447

ABSTRACT:
ABrucellabacterium is modified by partial or complete deletion of the pgm gene, rendering the bacterium incapable of synthesizing a key enzyme in the metabolism of bacterial sugars. A live vaccine for immunization, prophylaxis or treatment of brucellosis comprises such a bacterium, either lyophilized or in a pharmaceutical vehicle. Nucleotide sequence fragments having the aforementioned deletion are disclosed, with methods for making them.

REFERENCES:
patent: 5718903 (1998-02-01), Adams et al.
Comerci et al; Vector Development for the Expression of Foreign Proteins in the Vaccine StrainBrucella abortusS19; Infection and Immunity; Aug. 1998; pp. 3862-3866; vol. 66, No. 8; American Society for Microbiology.
Cornish-Bowden, A; Nomenclature for Incompletely Specified Bases in Nucleic Acid Sequences: Recommendations 1984; Nucleic Acid Research; 1985; pp. 3021-3030;vol. 13-No. 9; IRL Press Limited; Oxford, England.

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