Drug – bio-affecting and body treating compositions – Lymphokine
Patent
1982-12-14
1986-11-11
Warden, Robert J.
Drug, bio-affecting and body treating compositions
Lymphokine
435235, A61K 3912, C12N 700
Patent
active
046222227
ABSTRACT:
The invention relates to a process for the preparation of the lyophilized vaccine against duck hepatitis by using the attenuated hepatitis virus TN cultivated by Asplin.
According to the invention the virus deposited in the Strain Collection of the Hungarian Institute of Public Healts under No. 00220 is injected into the allantoic cavity of embryonated SPF-hen's eggs, the eggs are incubated at a temperature of about 37.degree. C., the embryos died between 24 and 96 hours are collected, homogenized with a physiological saline solution, antibiotics are added to the pure suspension, and after the addition of protective and skeleton forming agents the sterile virus material is lyophilized in a manner known per se.
The lyophilized vaccine can be stored at a temperature of +4.degree. C. for one year in contradiction to the known liquid vaccine storable in freezed state at a temperature of -20.degree. C. for half a year and which has to be used within 7 days from the delivery (with a storage temperature of +4.degree. C.). The titre of the vaccine prepared according to the invention is at least tenfold of that of the known vaccine, thus much less embryonated eggs are used for the process according to the invention what means cost saving. Due to the lyophilization the vaccine can be transported to far destinations, too, what was not possible at the known liquid product stored in freezed state.
REFERENCES:
patent: 4302444 (1981-11-01), Baxendale
Asplin, F. D., An Attenuated Strain of Duck Hepatitis Virus, The Veterinary Record, Dec. 27, 1958, pp. 1226-1230.
Biological Abstracts 73:61184, (1982).
Biological Abstracts 72:24311, (1981).
C A B Abstracts I0040-67195.
Hwang et al., Avian Diseases, 1964, 19,8, pp. 264-268.
Zvbtsova, State Control Inst. for Vet. Preparations, Zvenigorodsk oe Shosse 5, Moscow D-22.
Asplin F. D. and McLauchlan J. D.: Vet. Rec. 1954, 66, p. 456.
Asplin F. D., Vet. Rec., 1956, 68, p. 412.
Horvath Erzsebet
Tollas Gabor
Dubno Herbert
Foley Shawn P.
Phylaxia Oltoanyagtermelo Vallalat
Ross Karl F.
Warden Robert J.
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