Process for the preparation of a glucose-free hemoglobin standar

Details Process for the preparation of a glucose-free hemoglobin standar Process for the preparation of a glucose-free hemoglobin standar

1984-03-26

1986-05-20

Wallen, Thomas

Chemistry: analytical and immunological testing

Composition for standardization, calibration, simulation,...

Protein or peptide standard or control

436 16, 436 17, 436 18, 424101, 435 2, 435 25, 435 26, 435188, G01N 3100, A01N 102, C12Q 126, C12N 996

Patent

active

045901643

ABSTRACT:
A process for the preparation of a glucose-free glycosylated hemoglobin standard in which hemoglobin with a known content of glycosylated hemoglobin HbA.sub.Ia+b+c is converted to cyanmethemoglobin by means of a K.sub.3 (FeCN).sub.6 /KCN solution, stabilized with a sodium phosphate-cyanide buffer and preserved by lyophilizing at -40.degree. C. with a subsequent increase in temperature to -20.degree. C. under a high vacuum and stored at this temperature with the exclusion of light, whereupon the glucose-free glycosylated hemoglobin standard may be reconstituted by the addition of an aqueous medium.

REFERENCES:
patent: 3607695 (1971-09-01), Pfizer
patent: 3964865 (1976-06-01), Das
patent: 4465774 (1984-08-01), Huang et al.
patent: 4485174 (1984-11-01), Chiang et al.

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