Process for the preparation of 5'-ribonucleotides

Details Process for the preparation of 5'-ribonucleotides Process for the preparation of 5'-ribonucleotides

1985-08-12

1987-03-10

Naff, David M.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435177, 435180, C12P 1930, C12N 1102, C12N 1108

Patent

active

046491112

ABSTRACT:
Ribonucleic acid in a mixture of crude nucleic acids is selectively hydrolyzed with 5'-phosphodiesterase to 5'-ribonucleotides without hydrolyzing desoxyribonucleic acid in the mixture. Selective hydrolysis is carrying out by contacting the crude nucleic acid mixture with 5'-phosphodiesterase immobilized on a polymer carrier. The crude mixture of nucleic acids is preferably obtained by aqueous extraction of microorganisms that have previously been extracted with ammonia and a lower alcohol to remove lipids. The polymer carrier is preferably a copolymer of glycidylmethacrylate, allylglycidylether, methacrylamide and methylene-bis-methacrylamide.

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