Process for the preparation and purification of peptides

Chemistry: natural resins or derivatives; peptides or proteins; – Peptides of 3 to 100 amino acid residues – Synthesis of peptides

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530307, 530311, C07K 100

Patent

active

046237160

ABSTRACT:
Peptides, such as calcitonin, having two cysteine residues connected by a disulfide bond are prepared by a process which includes the step of cyclizing the cysteines at a pH of 8.5 to 9.0, and a concentration of 0.4 to 1.8 mg of peptide per ml of solution. These novel conditions produce rapid cyclization with an unforeseen improvement in yield.

REFERENCES:
patent: 3929758 (1975-12-01), Hughes et al.
patent: 4033940 (1977-07-01), Hughes et al.
patent: 4212795 (1980-07-01), Hughes et al.

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