Process for the physical depolymerization of...

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Reexamination Certificate

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C536S021000, C536S053000, C204S157600, C204S157630

Reexamination Certificate

active

07091337

ABSTRACT:
The invention relates to a process for the depolymerization of glycosaminoglycanes characterized by the use of high-energy radiation in the presence of an organic compound selected from the group consisting of ethers, alcohols, aldehydes, amides and formic acid. The invention also relates to intermediate depolymerized heparin having a molecular weight of between 1,000 and 5,500, absorbance at 400 nm below 0.300 and ratio SO3−/COO−equal to or higher than in the starting heparin. The intermediate depolymerized heparin can be dissolved in a buffer solution and fractionated by gel permeation for obtaining the desired molecular weight.

REFERENCES:
patent: 4987222 (1991-01-01), De Ambrosi et al.
patent: 6383344 (2002-05-01), Miller et al.
patent: 0269937 (1991-02-01), None
patent: WO 90/04607 (1990-05-01), None
patent: WO 90/04607 (1990-05-01), None
patent: WO 00/69444 (2000-11-01), None
Baquey C. et al., “ESR Study of Gamma-Irradiated Sodium Heparinate”Radiation Research, 70:82-90 (1997).
Ershov B.G., “Radiochemical Destruction of Cellulose and Other Polysaccharides”,Uspekhi Khimiki, 67:353-375 (1998).
Jooyandeh F. et al., “Chemical Effects of Gamma-Irradiation of Aqueous Solutions of Heparin and Keratan Sulphate”,Radiation Research, 45:455-461 (1971).

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