Process for the overexpression of dehydrogenases

Details Process for the overexpression of dehydrogenases Process for the overexpression of dehydrogenases

2003-01-31

2008-08-26

Pak, Yong D (Department: 1652)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing oxygen-containing organic compound

C435S190000, C435S004000, C435S006120, C435S026000, C435S069100, C435S071100, C435S252300, C435S320100, C435S440000, C536S023100, C536S023200

Reexamination Certificate

active

07416866

ABSTRACT:
A process for the overexpression of dehydrogenases, especially for the overexpression of Δ1-dehydrogenases, in particular for the overexpression of 3-keto steroid-Δ1-dehydrogenases, as well as for the bacteria, plasmids and DNA sequences that can be used for the overexpression, is described.

REFERENCES:
Branden et al. Introduction to Protein Structure, Garland Publishing Inc., New York, p. 247, 1991.
Wang et al. Heterologous gene expression inBacillus subtilis. Biotechnology. 1992;22:63-104.
Molnar et al.Molecular cloning, expression inStreptomyces lividans, and analysis of a gene cluster from Arthrobacter simplex encoding 3-ketosteroid-delta 1-dehydrogenase, 3-ketosteroid-delta 5-isomerase and a hypothetical regulatory protein. Mol Microb ., (1995) 15(5), 895-905.
Molnar et al. NCBI database D37969. 1999.

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