Process for the microbial production of alcoholoxidase

Details Process for the microbial production of alcoholoxidase Process for the microbial production of alcoholoxidase

1979-07-09

1981-02-10

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

435 25, 435 28, 435147, 435168, 435930, C12N 904

Patent

active

042502616

ABSTRACT:
A method of producing alcohol oxidase wherein Hansenula polymorpha yeast is cultivated in a nutrient broth containing inorganic nutrient salts of which either the phosphate, nitrate or potassium is present in a cell-growth-limiting concentration at a temperature of 25.degree. to 45.degree. C. and a pH of 4.0 to 6.0 with oxygen being supplied as air for a mixture of air and oxygen. According to the invention, an organic substrate consisting of glycerin, sorbite or xylose, noncatabolite-repressive to alcohol-oxidase is added to the broth in a concentration of 0.1 to 1.0% in a nutrient solution at a rate of 0.03 to 0.15 .sup.-1.

REFERENCES:
patent: 4168201 (1979-09-01), Wegner
Reuss et al., Chemie-Ingenieur-Technik, vol. 46, No. 16, pp. 669-676, (1974).
Barman, Enzyme Handbook, Supplement I, 1974, pp. 70-71.

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