Process for the manufacture of thrombin inhibitors

Details Process for the manufacture of thrombin inhibitors Process for the manufacture of thrombin inhibitors

1995-03-03

1998-03-17

LeGuyader, John

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4351723, 4352523, 43525231, 43525421, 4353201, 536 235, C12N 1515, C12N 1575, C12N 1581

Patent

active

057285497

ABSTRACT:
The invention relates to DNA sequences that code for the amino acid sequence of the thrombin inhibitor hirudin, hybrid vectors containing such DNA sequences, host cells transformed with such hybrid vectors, novel polypeptides with thrombin-inhibiting activity produced from such transformed host cells, processes for the manufacture of these DNA sequences, hybrid vectors and transformed host cells, and processes for the manufacture of these thrombin-inhibitors using the transformed host cells. The hirudin compounds that can be produced according to the invention have valuable pharmacological properties.

REFERENCES:
patent: 3432596 (1969-03-01), Markwardt et al.
patent: 4517294 (1985-05-01), Bock et al.
patent: 4654302 (1987-03-01), Fritz et al.
patent: 4704362 (1987-11-01), Itakura et al.
patent: 5162208 (1992-11-01), Lemoine et al.
Ganesar et al. Genetics & Biotechnology of Bacilli, Academic Press, Orlando, FL, 1984. p. 161.
Bagdy et al., "Large Scale Preparation of Hirudin", Thrombosis Research 2:229-238 (1973).
Bagdy et al., "Protcolytic Enzymes", Methods Enzym., XLV:669-678 (1976).
Bergmann et al., "Chemical Synthesis and Expression of a Gene Coding for Hirudin, the Thrombin-Specific Inhibitor from the Leech Hirudo medicinalis", Biol. Chem. Hoppe Seyler's 367:731-740 (1986).
Burleigh et al., "Development of biosynthetic somatomedin-C/IGF-I as a product for cell culture", Americ. Biol. Lab. 48 and 50 (1986).
Chang, "The functional domain of hirudin, a thrombin-specific inhibitor", FEBS 1044, 164:307-313 (1983).
Dodt et al., "The Complete amino acid sequence of hirudin, a thrombin specific inhibitor", Fed. of European Bio. Societies 165:180-184 (1984).
Grosjean et al., "Preferential codon usage in prokaryotic genes: the optimal codon-anticodon interaction energy and the selective codon usage in efficiently expressed genes". Gene 18:199-209 (1982).
Guarente et al., "A Technique for Expressing Eukaryotic Genes in Bacteria", Science 209:1428-1430 (1980).
Gueriguian et al., "Insulins, Growth Hormone, and Recombinant DNA Technology", Raven Press N.Y. 39-45 (1981).
Ikehara et al., "Sythesis of a gene for human growth hormone and its expression in Escherichia coli", Proc. Natl. Acad. Sci. USA 81:5956-5960 (1984).
Lahm et al., "Characterization of Recombinant Human Interleukin-2 with micromethods", Journal of Chromatography 326:357-361 (1985).
Loffler et al., "Lehrbuch der Medizinischen Biochemie und Pathobiochemie fur studierende der Medizin und Arzte", Physiologische Chemie 718-719 (1985).
Maniatis et al., "Molecular Cloning: A Laboratory Manual", Cold Spring Harbor Laboratory 226-227, 403-404, 412-413, and 422 (1982).
Markwardt et al., "Pharmacological Studies on the Antithrombotic Action of Hirudin in Experimental Animals", Thromb Haemostas 47:226-229 (1982).
Miller, C. G., "Protein Degradation and Proteolytic Modification", Escherichia coli and Salmonella Thyphimurium Cellular and Molecular Biology 1:687 (1987).
Miyanohara et al., "Expression of hepatitis B surface antigen gene in yeast", Prod. Natl. Acad. Sci. USA 80:1-5 (1983).
Palva et al., "Secretion of interferon by Bacillus subtilis", Gene 22:229-235 (1983).
Riehl-Bellon et al., "Purification and Biochemical Characterization of Recombinant Hirudin Produced by Saccharomyces cerevisiae", Biochemistry 28:2941-2949 (1989).
Schoemaker et al., "Examination of calf prochymosin accumulation in Escherichia coli: disulphide linkages are a structural component of prochymosin-containing inclusion bodies", The EMBO Journal 4:775-780 (9185).
Seeburg et al., "Efficient Bacterial Expression of Bovine and Porcine Growth Hormones", DNA 2:37-45 (1983).
Sherman et al., "Methionine or Not Methionine at the Beginning of a Protein", Bio Essays 3:27-31 (1985).
Staehelin et al., "Purification and Characterization of Recombinant Human Leukocyte Interferon (IFLrA) with Monoclonal Antibodies", Journal of Biological Chemistry 256:9750-9754 (1981).
Tuggle et al., "Glutathione Reductase Is Not Required for Maintenance of Reduced Glutathione in Escherichia coli K-12", Journal of Bacteriology 162:448-450 (1985).
Valenzuela et al., "Synthesis and assembly of hepatitis B virus surface antigen particles in yeast", Nature 298:347-350 (1982).
Vogt, V. M., "Purification and Properties of an Aminopeptidase from Escherichia coli", Journal of Biological Chemistry 245:4760-4769 (1970).
Walsmann et al., "Biochemische und pharmakologische Aspekte des Thrombininhibitors Hirundin", Die Pharmazie 36:653-660 (1981).
Wetzel et al., "Expression in Escherichia coli of a chemically synthesized gene for a mini-C analog of human proinsulin", Gene 16:63-71 (1981).
Wetzel et al., "The Peptides", Eds. Gross et al., Academic Press, N.Y. 5:32 (1983).
Williams et al., "Cytoplasmic Inclusion Bodies in Escherichia coli Producing Biosynthetic Human Insulin Proteins", Science 215:687-689 (1982).
Winkler et al., "Purification and Characterization of Recombinant Urokinase From Escherichia coli", Biotechnology 3:990-1000 (1985).
Winnacher, "From Genes to Clones: Introduction to Gene Technology", WCH Publishers, N.Y. 293 (1987).

Affiliated with

Also associated with

Rating

Process for the manufacture of thrombin inhibitors does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Process for the manufacture of thrombin inhibitors, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for the manufacture of thrombin inhibitors will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-956611