Process for the manufacture of pyruvate oxidase, and analytical

Details Process for the manufacture of pyruvate oxidase, and analytical Process for the manufacture of pyruvate oxidase, and analytical

1979-03-21

1981-01-20

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435 16, 435 17, 435 19, 435192, 435810, 435822, 435885, C12Q 126, C12N 908

Patent

active

042463424

ABSTRACT:
Pyruvate oxidase can be produced by culturing Pediococcus sp. B-0667, Streptococcus sp. B-0668, Aerococcus viridans IFO-12219 or Aerococcus viridans IFO-12317. It is useful for analysis for pyruvic acid, because it catalyzes the reaction of pyruvic acid, phosphate and oxygen to form acetylphosphate, carbon dioxide and hydrogen peroxide. A kit containing the various reagents for such analysis is also provided by this invention.

REFERENCES:
Dixon et al., Enzymes, 2nd Edition, 1964, pp. 684-685.
Colowick et al., editors, Methods in Enzymology, vol. 1, pp. 482-490 (1955).

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