Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide
Patent
1980-01-07
1981-09-29
Rosen, Sam
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Enzymatic production of a protein or polypeptide
435241, C12P 2100, C12N 502
Patent
active
RE0307530
ABSTRACT:
A new system of serially culturing human anterior pituitary gland cells in a new nutrient medium to produce large amounts of human growth hormone. Since only human growth hormone can be used to treat growth deficiencies in man, there is a great demand for the hormone, which is in relative short supply since only 2 to 3 mgs. of the human growth hormone can be extracted from one human pituitary gland obtained at autopsy. Using the new system of culture and new nutrient media, the human pituitary cells can be grown in vitro to produce in approximately three weeks, more than 20 times the amount of extractable growth hormone than that which was originally present in the original tissue now used for extracting the 2 to 3 mgs. of the hormone.
REFERENCES:
patent: 3073746 (1963-01-01), Thompson et al.
The Merck Index-Ninth Edition, (1976), p. 7290.
Betteridge et al.-Chem. Abst., vol. 80, (1974), p. 44148k.
Anderson John A.
Narasimhan Mandayam J.
Rosen Sam
The Regents of the University of Minnesota
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