Process for the isolation of basement membrane proteins from hum

Details Process for the isolation of basement membrane proteins from hum Process for the isolation of basement membrane proteins from hum

1988-03-11

1992-09-15

Kepplinger, Esther L.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 71, 435 23, 435184, 435803, 435820, 435961, 435962, 436 63, 436177, 436811, 436825, 530412, G01N 33567, C12N 999

Patent

active

051477823

ABSTRACT:
Basement membrane proteins are isolated as functioning proteins in relatively large amounts from human or animal tissues in aqueous solution in the presence of a chelating agent. It is possible to use these proteins to obtain highly specific antibodies which are used for the immunological determination of these proteins.

REFERENCES:
patent: 4628027 (1986-12-01), Gay
Dziadek et al., Biological Abstracts, vol. 80(5), 1985, Abstract No. 40294.
Dziadek et al., "Purification and Tissue Distribution of a Small Protein (BM-40) Extracted From a Basement Membrane Tumor", European Journal of Biochemistry 161:455-464 (1986).
Ohno et al., "Isolation of Laminin from Human Placental Basement Membranes: Amnion, Chorion and Chorionic Microvessels", Biochemical and Biophysical Research Communications, 112:1091-1098 (1983).
Paulsson et al., "Purification and Structural Characterization of Intact and Fragmented Nidogen Obtained from a Tumor Basement Membrane", European Journal of Biochemistry 156:467-478 (1986).
Risteli et al, "Isolation and Characterization of Pepsin Fragments of Laminin from Human Placental and Renal Basement Membranes" Biochemistry Journal 193:749-755 (1980).
Timpl et al., "Laminin--A Glycoprotein from Basement Membranes", The Journal of Biological Chemistry 254:9933-9937 (1979).
Wewer et al, "Human Laminin Islated in a Nearly Intact, Biologically Active Form from Placenta by Limited Proteolysis", The Journal of Biological Chemistry 258:12654-12660 (1983).
Vincent et al., Biological Abstracts, vol. 72(1), Abstract No. 2537, 1981.
Victoria et al., Biological Abstracts, vol. 72(12), Abstract No. 78502, 1981.

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