Process for the isolation and the characterization of a gene enz

Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part

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4353201, 435419, 435468, 800288, 8003172, 8003173, A01H 500, C12N 514, C12N 1582

Patent

active

060606452

ABSTRACT:
Transformed plants resistant to phenmedipham, which are resistant by having been transformed with a purified and isolated carbamate hydrolase gene. The carbamate hydrolase enzyme produced thereby is responsible for the cleavage of the carbamate bond between the benzene rings of phenmedipham. The invention includes the transformed plants, progeny thereof, transformed plant cells, and method of producing the transformed plants and transformed plant cells, which are resistant to phenmedipham. The invention further includes plasmids comprising the carbamate hydrolase gene capable of inactivating phenmedipham.

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Stam M, et al. "The silence of genes in transgenic plants." Ann. Bot. 79: 3-12, 1997.
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Rothstein SJ, et al. "Promoter cassettes, antibiotic-resistance genes, and vectors for plant transformation." Gene 53: 153-161, 1987.
C.O. Knowles, et al. "Microbial Degradation of Carbamate Pesticides Desmedipham, Phenmendipham, Promecarb, and Propamocarb", Bull. Environm. Contam. Toxicol., 27, 529-533 (1981).
David N. Radin, et al., "Herbicide-tolerant tobacco mutants selected in situ and recovered via regeneration from cell structure", Genet. Res. Camb., (1978), 32, pp. 85-89.

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